Download e-book for kindle: Analysis of Genetic Variation in Animals by M. Caliskan

By M. Caliskan

ISBN-10: 9535100939

ISBN-13: 9789535100935

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84 indicated high level of genetic variability of Albanian sheep breed. , 2010). 61 alleles per breed. , 2009). 75. , 2009). , 2008). Estimates of observed heterozygosity confirm the high level of diversity evidenced in the Albanian sheep breeds. 72. , 2003). The comparison of average observed and expected heterozygosity values did not show great differences between breeds. All breeds showed smaller observed than expected heterozygosities (Table 3). Bardhoka and Shkodrane showed on 3 loci out of 31, significant deviation from Hardy Weinberg proportion, but Ruda showed no deviations from Hardy Weinberg proportion.

They can be highlighted through various techniques such as: RFLP (Restriction Fragment Length Polymorphism) analysis, SSCP (Single-Strand Conformation Polymorphism), genotyping fluorescent labeled fragments or sequencing. Microsatellites are useful markers for population genetic studies because they offer advantages which are particularly appropriate for conservation projects: they are widely available and exhibit a high degree of polymorphism. In addition, it is assumed they are neutral to selection, since the observed genetic diversity is constituted as the consequence of two forces: genetic drift and mutation.

Hogg, F. C. Buchanan, A. M. Crawford, and F. W. Allendorf. 1995. Microsatellite evolution in congeneric mammals: domestic and bighorn sheep. Molecular Biology and Evolution 12: 1106. Francisco, L. ; A. A. Langsten, C. S. Mellersh, C. L. Neal, and E. A. Ostrander. 1996. A class of highly polymorphic tetranucleotide repeats for canine genetic mapping. Mammalian Genome 7: 359-362. Freeman, A. R. et al. 2004. Admixture and diversity in West African cattle populations. Molecular Ecology 13: 3477-3487.

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Analysis of Genetic Variation in Animals by M. Caliskan

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